91˿Ƶ

Leica STELLARIS 8 - STED-FLIM

91˿Ƶ

Stellaris 8 Confocal Microscope

DMi8 motorized and automated inverted microscope stand

STED microscopy and lifetime‐based imaging with tauSense and FALCON (TCSPC‐based FLIM)

Leica STELLARIS 8 - STED-FLIMFeatures:

  • 4D Imaging, multi-color, z-stack, timelapse
  • DIC
  • Navigator mode for easy overview and tiling
  • LIGHTNING module for enhanced resolution
  • STED
  • FLIM
  • Excitation and emission lambda scans to characterize sample or for spectral unmixing
  • Stage‐top incubation system

Light Sources:

  • LED3 white light source for epi-fluorescence visualization
  • 405nm laser
  • Tunable White Light Laser (WLL) with 350 laser lines 440‐790nm (pulsed with 80MHz repetition rate)
  • 775nm depletion laser for 3D STED microscopy

Detectors:

  • 3 HyD S detectors
  • 2 HyD X detectors

Objectives:

PL APO 10x/0.40 CS2

  • FWD: 2.56mm
  • Coverglass: 0.17mm
  • Immersion: dry

PL APO 20x/0.75 CS2

  • FWD: 0.62mm
  • Coverglass: 0.17mm
  • Immersion: dry

PL APO 93x/1.30 GLY motCORR STED WHITE

  • FWD: 0.3mm
  • Coverglass: 0.14‐0.17mm
  • Motorized correction collar (motCORR)
  • Immersion: Glycerol

PL APO 100x/1.40 oil STED WHITE

  • FWD: 0.13mm
  • Coverglass: 0.17mm
  • Immersion: oil

Fluorescence Cubes:

15525338 Filter cube LED 405

  • Excitation: 405/60
  • Emission: 470/40

15525314 Filter Cube GFP

  • Excitation: BP 470/40
  • Emission: BP 525/50

15525303 Filter Cube RHOD LP

  • Excitation: BP 540/45
  • Emission: LP 590

Location:

  • Located in Bellini Building, Room 151.

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