91Ë¿¹ÏÊÓƵ

Imaging

91Ë¿¹ÏÊÓƵ

NIKON CSU-X1 Spinning Disk Confocal

Applications: Intravital Imaging, 4D Confocal, Fast Kinetics, Widefield Fluorescence, Brightfield

Excitation:

- Four lasers lines for confocal imaging: 405nm, 488nm, 561nm, 640nm.
- Four LED lines for widefield fluorescence: 385nm, 488nm, 561nm, 621nm.
- Brightfield illumination.

Emission:

- Four single chanel emission filters: 455/50nm, 525/36nm, 605/52nm, 705/72nm.
- One quad chanels filter for high speed acquisition: DAPI/FITC/TRITC/CY5.

Detection:

- Confocal: Photometrics Prime BSI Express sCMOS Camera. 2048x2048, 95fps@11-bits/43fps@16-bits.
- Widefield and Brigthfield: Nikon Digital Sight 10, Color and Monochrome mode, 25 mm FOV, 23.9 megapixel.

Objectives:Ìý10X/0.45 air, 20X/0.75 air, 16X/0.8 water dipping, 20X/1.0 water, 60X/1.42 oil.

Located in a biosafety level 2 room that include a BSC.

Image processing and analysis: Nikon imaging software (NIS) workstation and Bitplane Imaris 9.1.2

Ìý

LEICA SP8 Laser Scanning Confocal and Multiphoton

Applications: 4D Confocal and Multiphoton Imaging, Spectral imaging, FLIM, FRET, FRAP modules, SHG

Excitation:

-Ìý Four solid state laser lines: 448 nm, 488 nm, 552 nm,
Ìý 638 nm
-Ìý One tunable IR two-photons laser: 680-1080 nm

Detection:

-Ìý Two internal HyDs (high sensitivity detectors)
-Ìý Two internal PMTs
-Ìý One transmitted light PMT
-Ìý Two reflected light HyD
-Ìý One brightfield detector (Dodt gradient)

Objectives: 4x to 63x (including air, oil and multi-immersion)

Environmental control:ÌýCO2, temperature, humidity.

Image processing and analysis: Leica LAS XÌýand Bitplane Imaris 9.1.2

Ìý Ìý

Perfusion system for live tissue imaging
Perfusion system for live tissue imaging
Ìý Ìý
Fly eye autofluorescence
Fly eye autofluorescence
Second Harmonic and two-photon fluorescence on human aorta
Second Harmonic and two-photon fluorescence on human aorta

PerkinElmer High Content Microscopes

Applications: Fully automated fluorescence and brightfield imaging of microplates (fixed or live samples) and standard slides. Automated high content image analysis.

    Opera Phenix Plus automated microscope:

    • From 6 well to 1536 well plates and standard slides.
    • Perkin Elmer Opera Phenix Plus and Cell Explorer Station
      Perkin Elmer Opera Phenix Plus and Cell Explorer Station
      5x, 10x, 20x air objective
    • 20X,Ìý40x and 60X high numerical aperture water immersionÌýobjectives
    • Brightfield, Digital Phase Contrast, Widefield or confocal (lens spinning disk)
    • Five laser lines (375nm, 425nm, 488nm, 561nm, 640nm)
    • FourteenÌýemission filters for wide or narrow emission window
    • Four sCMOS 16 bit camera (2160px X 2160px)
    • Fast frame-rate imaging capacity (up to 105 fps)
    • Internal liquid handling capacity for fast kinetic acquisition (i.e., Calcium imaging)
    • Connected to a plate handler, a liquid handler, a plate washer, an incubator and a bar scanner.
    • Optional environmental control chamber (CO2, Temperature, Humidity)

    Operetta automated microscope:

    • From 6 well to 1536 well plates and standard slides
    • Perkin Elmer Operetta automated microscope
      Perkin Elmer Operetta automated microscope
      2x, 10x, 20x and 40x objectives
    • A wide range of filter combinations
    • Optional confocality (spinning disk)
    • Optional environmental control chamber
    • 300W xenon lamp

    Browser-based image analysis software (Columbus)


    Stereo-microscope (Leica M165)sterio microscope

    -Ìý Color camera 5Mp (Leica DFC450C)
    -Ìý Epifluorescence (120W Mercury Halide lamp)
    -Ìý Filter: green (GFP), red (RhodB), YFP, mCherry
    -Ìý Reflected and Transmitted light set-up
    -Ìý Leica LAS X acquisition software with multi focus and overlay module

    Upright microscope (Leica DM1000)upright microscope

    -Ìý Color camera 1.4Mp (Leica DFC310FX)
    -Ìý Epifluorescence, Brightfield (120W Mercury Halide lamp)
    -Ìý Filter: blue (A4), red (N2.1), green (L5)
    -Ìý Leica LAS X acquisition software

    Inverted microscope (Leica DMI 6000)inverted microscope

    -Ìý Monochrome camera 2Mp (Leica DFC345FX)
    -Ìý Epifluorescence, DIC, IMC (120W Mercury Halide lamp)
    -Ìý Filter sets for DAPI, CFP, GFP and TxRed
    -Ìý Leica LAS X acquisition software
    -ÌýÌýEppendorf piezo system for microinjection/manipulation

    Back to top